2018-06-18 14:57:102025-05-14 13:56:11
locus
BSU04970
BSU_04970
geneLength
987
990
outlinks
bsu
BSU04970
BSU_04970
Gene
Coordinates
544,022 → 545,011
544,022 545,011
The protein
Catalyzed reaction/ biological activity
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) [Pubmed|18305117]
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and meso-diaminopimelic acid) [Pubmed|18305117]
degradation of gamma-polyglutamic acid [pubmed|29458655]
The protein
Protein family
nlpC/p60 family (according to Swiss-Prot)
[SW|Peptidase C40 family] (according to UniProt)
The protein
[SW|Domains]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid [Pubmed|18305117]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507], cleaves the bond between D--glutamate and meso-diaminopimelic acid [Pubmed|18305117]
Biological materials
Mutant
BKE04970 (Δ[[gene|cwlT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKK04970 (Δ[[gene|cwlT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKE04970 ([[gene|cwlT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKK04970 ([[gene|cwlT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
References
The protein
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [[protein|IseA]] [pubmed|29458655]